Zhang Y P, Verhaagen J, Hamers F P, Gispen W H
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Sheng Li Xue Bao. 1995 Feb;47(1):38-44.
Using in-situ hybridization method. We studied B-50 (GAP-43) mRNA expression following lesion of olfactory bulb mitral cells in rats. Expression of B-50 mRNA in approximately 40% of the mitral cells was upregulated in response to transection of their axons in the lateral olfactory tract (LOT). Enhanced expressin persisted for 10 days postlesion but than declined to control levels by 4 weeks after the lesion. A large proportion of the mitral cells gradually degenerated subsequent to LOT transection. Thus, a subpopulation of mitral cells maintain their ability to upregulate B-50, a protein characteristic of growing axons, but enhanced B-50 expression is not accompanied by regeneration of the severed LOT.
采用原位杂交法，我们研究了大鼠嗅球二尖瓣细胞损伤后B - 50（GAP - 43）mRNA的表达情况。在外侧嗅束（LOT）中，约40%的二尖瓣细胞的轴突被横断后，其B - 50 mRNA的表达上调。损伤后10天内，B - 50 mRNA表达增强持续存在，但损伤4周后降至对照水平。LOT横断后，大部分二尖瓣细胞逐渐退化。因此，二尖瓣细胞的一个亚群保持上调B - 50的能力，B - 50是生长轴突的一种蛋白质特征，但B - 50表达增强并未伴随切断的LOT再生。
