Gasser R B, Chilton N B
Department of Veterinary Science, University of Melbourne, Werribee, Vic., Australia.
Acta Trop. 1995 Mar;59(1):31-40. doi: 10.1016/0001-706x(94)00085-f.
Seven species of taeniid cestode (Echinococcus granulosus. E. multilocularis, Taenia hydatigena, T. ovis, T. pisiformis, T. multiceps and T. serialis) were characterised using a polymerase chain reaction-based restriction fragment length polymorphism technique (PCR-RFLP). The second internal transcribed spacer of ribosomal DNA (ITS2) was amplified from various geographical isolates of each of the seven species, digested separately with four restriction endonucleases and the fragments were separated by conventional agarose gel electrophoresis. PCR-RFLP produced characteristic patterns for each taeniid species examined. No variation in RFLP patterns was observed among different isolates of E. multilocularis and the species of Taenia, but distinct intraspecific variation was detected in E. granulosus. The present study indicates the usefulness of the PCR-RFLP of ITS2 for systematic, epidemiological and diagnostic purposes.
利用基于聚合酶链反应的限制性片段长度多态性技术（PCR-RFLP）对7种带绦虫（细粒棘球绦虫、多房棘球绦虫、泡状带绦虫、绵羊带绦虫、豆状带绦虫、多头带绦虫和连续带绦虫）进行了鉴定。从这7个物种的不同地理分离株中扩增核糖体DNA的第二内部转录间隔区（ITS2），分别用4种限制性内切酶进行消化，并用常规琼脂糖凝胶电泳分离片段。PCR-RFLP为所检测的每种带绦虫物种产生了特征性图谱。在多房棘球绦虫和带绦虫属的不同分离株之间未观察到RFLP图谱的差异，但在细粒棘球绦虫中检测到明显的种内变异。本研究表明ITS2的PCR-RFLP在系统学、流行病学和诊断方面具有实用性。
