Bowles J, McManus D P
Molecular Helminthology Laboratory, Queensland Institute of Medical Research, Brisbane, Australia.
Mol Biochem Parasitol. 1993 Feb;57(2):231-9. doi: 10.1016/0166-6851(93)90199-8.
Echinococcus species and genetically distinct strains of Echinococcus granulosus can be rapidly and reliably identified using a polymerase chain reaction (PCR)-linked restriction fragment length polymorphism (RFLP) method which surveys the sequence of a rapidly evolving region of the ribosomal DNA (rDNA) unit. Internal transcribed spacer 1 (ITS1) of the rDNA repeat was amplified from various isolates and the product was digested with one of a number of 4-base cutting restriction enzymes. Characteristic patterns were produced when samples within various species and strain groups were analysed. This method offers an objective, simple, highly sensitive and rapid approach for the discrimination of Echinococcus isolates and for study of other parasite complexes.
使用聚合酶链反应（PCR）连接的限制性片段长度多态性（RFLP）方法，可以快速、可靠地鉴定棘球绦虫属物种和细粒棘球绦虫的基因不同菌株，该方法可检测核糖体DNA（rDNA）单元快速进化区域的序列。从各种分离株中扩增rDNA重复序列的内部转录间隔区1（ITS1），并用多种4碱基切割限制性内切酶之一消化产物。对不同物种和菌株组内的样本进行分析时，会产生特征性图谱。该方法为棘球绦虫分离株的鉴别以及其他寄生虫复合体的研究提供了一种客观、简单、高度灵敏且快速的方法。
