Wang K, Gan L, Boysen C, Hood L
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
Anal Biochem. 1995 Mar 20;226(1):85-90. doi: 10.1006/abio.1995.1195.
A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96-well membrane-bottomed assay plate. With this method, 50% of the DNA is recovered routinely using Sephacryl-500HR as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations.
