Gomm J J, Browne P J, Coope R C, Liu Q Y, Buluwela L, Coombes R C
Department of Medical Oncology, Charing Cross and Westminster Medical School, University of London, United Kingdom.
Anal Biochem. 1995 Mar 20;226(1):91-9. doi: 10.1006/abio.1995.1196.
Monoclonal antibodies to epithelial membrane antigen and common acute lymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were then used to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 x 10(6) epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment.
将上皮膜抗原和常见急性淋巴细胞白血病抗原的单克隆抗体与包被有二抗的磁性微球结合。然后，这些特异性抗体/磁珠复合物通过磁分离直接从正常乳腺类器官细胞制剂中分离纯化上皮细胞和肌上皮细胞。选择了近乎均一的细胞群体，每个类器官制剂可获得10 - 20×10⁶个上皮细胞和肌上皮细胞。重复的纯化步骤几乎可完全去除用于RNA研究的肌上皮细胞。在合适的限定培养基中对分离的细胞群体进行组织培养，进一步确保了细胞类型的纯度，且不受磁珠附着的影响。
