Casarotti V T, Gallo C R, Camargo R
Departamento de Ciência e Tecnologia Agroindustrial da Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP).
Arch Latinoam Nutr. 1994 Sep;44(3):158-63.
Samples of raw milk, pasteurized C type milk and minas frescal cheese were analyzed for the presence of Listeria monocytogenes by a method developed for dairy products by Lovett (1) and by Lovett & Hitchins (2). A total of 20 samples of each product and from different commercial brands were analyzed, obtained from various retail stores in Piracicaba, SP. According to the pattern of sampling and methodology adopted, none of the samples of the aforesaid products was positive for the presence of L. monocytogenes or another species of the genus. It was possible to observe differences in the degree of selectivity of the selective media utilized, notwithstanding the LPM agar showing to be superior to MMA. Based on the literature and on the result obtained, becomes evident the need of a more suitable method for the detection and isolation of L. monocytogenes from foods, even when the patogen appears in low numbers, with purpose to obtain rapid, reliable and reproducible results.
采用Lovett(1)以及Lovett和Hitchins(2)为乳制品开发的方法,对生牛奶、巴氏杀菌C型牛奶和米纳斯弗雷斯卡尔奶酪样本进行了单核细胞增生李斯特菌检测。从圣保罗州皮拉西卡巴的多家零售店采集了每种产品的20个样本,这些样本来自不同的商业品牌。根据所采用的采样模式和方法,上述产品的样本均未检测出单核细胞增生李斯特菌或该属的其他物种呈阳性。尽管LPM琼脂显示优于MMA,但可以观察到所使用的选择性培养基的选择性程度存在差异。基于文献和所得结果,显而易见的是,即使病原菌数量很少,也需要一种更合适的方法来从食品中检测和分离单核细胞增生李斯特菌,以便获得快速、可靠和可重复的结果。