Moura S M, Destro M T, Franco B D
Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Int J Food Microbiol. 1993 Aug;19(3):229-37. doi: 10.1016/0168-1605(93)90080-z.
The present study evaluated the incidence of Listeria spp. in raw and pasteurized milk from a Brazilian dairy plant. Ten samples of each type of milk (raw types B and C and pasteurized types B and C) were collected monthly from October 1989 to September 1990 (except in December), comprising 440 samples (110 samples of each type of milk). The recovery of Listeria spp. was carried out using LPM (lithium chloride phenylethanol moxalactam) and MOX (modified Oxford) agars, after a two-step enrichment procedure in Listeria enrichment broth (LEB) and Fraser broth. Overall, 12.7% of raw milk samples, 0.9% of pasteurized milk samples and 6.8% of total of milk samples were positive for Listeria spp., while 9.5% of raw milk samples, none of the pasteurized milk samples and 4.8% of total milk samples, were positive for Listeria monocytogenes. Raw milk also contained L. innocua (9.5%), L. welshimeri (0.9%) and L. grayi (0.4%). Pasteurized milk contained only L. innocua (0.9%).
本研究评估了巴西一家乳制品厂的生牛奶和巴氏杀菌乳中李斯特菌属的发生率。从1989年10月至1990年9月（12月除外）每月采集每种类型牛奶的10个样本（生牛奶B型和C型以及巴氏杀菌牛奶B型和C型），共440个样本（每种类型牛奶110个样本）。在李斯特菌增菌肉汤（LEB）和弗雷泽肉汤中进行两步富集程序后，使用LPM（氯化锂苯乙醇莫西沙星）和MOX（改良牛津）琼脂进行李斯特菌属的分离。总体而言，12.7%的生牛奶样本、0.9%的巴氏杀菌乳样本以及6.8%的牛奶样本总数对李斯特菌属呈阳性，而9.5%的生牛奶样本、无巴氏杀菌乳样本以及4.8%的牛奶样本总数对产单核细胞李斯特菌呈阳性。生牛奶还含有无害李斯特菌（9.5%）、威氏李斯特菌（0.9%）和格氏李斯特菌（0.4%）。巴氏杀菌乳仅含有无害李斯特菌（0.9%）。
