van Rhee F, Kasprzyk A, Jamil A, Dickinson H, Lin F, Cross N C, Galvin M C, Goldman J M, Secker-Walker L M
LRF Centre for Adult Leukaemia, Royal Postgraduate Medical School, London.
Br J Haematol. 1995 May;90(1):225-8. doi: 10.1111/j.1365-2141.1995.tb03408.x.
The Philadelphia (Ph) chromosome is detected in leukaemia cells in approximately 20% of adults with acute lymphoblastic leukaemia (ALL). When treated with chemotherapy alone, Ph-positive ALL has a poor prognosis, and patients may benefit from bone marrow transplantation in first remission. Here we report a patient with chromosomally normal bone marrow, in all 60 cells analysed, who was found to have the p210-type BCR-ABL chimaeric transcript by RT/PCR. Fluorescence in situ hybridization was labelled cosmid probes for BCR and ABL showed the presence of BCR-ABL juxtaposition on a normal chromosome 22 in leukaemia cell metaphases. We conclude that molecular and cytogenetic methods should be used in conjunction to detect the BCR-ABL gene rearrangement in ALL.
在大约20%的成年急性淋巴细胞白血病（ALL）患者的白血病细胞中可检测到费城（Ph）染色体。单独接受化疗时，Ph阳性ALL的预后较差，患者在首次缓解时可能受益于骨髓移植。我们在此报告一名骨髓染色体正常的患者，在分析的所有60个细胞中，通过逆转录/聚合酶链反应（RT/PCR）发现其具有p210型BCR-ABL嵌合转录本。荧光原位杂交用针对BCR和ABL的黏粒探针标记，结果显示在白血病细胞中期的一条正常22号染色体上存在BCR-ABL并置。我们得出结论，应联合使用分子和细胞遗传学方法来检测ALL中的BCR-ABL基因重排。
