Poulain S, Daudignon A, Le Baron F, Bisiau H, Simon M, Duthilleul P
Département d'Hématologie-Immunologie-Cytogénétique, Centre Hospitalier de Valenciennes, France.
Cancer Genet Cytogenet. 1999 Jan 15;108(2):158-61. doi: 10.1016/s0165-4608(98)00082-x.
We report on a 67-year-old woman with acute lymphoblastic leukemia (ALL) who was supposed to have a variant Philadelphia (Ph) translocation identified by conventional cytogenetic techniques. Fluorescence in situ hybridization (FISH) analysis demonstrated the presence of an amplification of BCR-ABL rearrangement at locus 22q11. This is the first observation, to our knowledge, of a duplicated BCR-ABL chimeric gene within the derived chromosome 22 in ALL. Our observation supports the possibility of detecting a variant Ph chromosome at the single-cell level by FISH analysis.
我们报告了一名67岁的急性淋巴细胞白血病（ALL）女性患者，通过传统细胞遗传学技术检测到其存在一种变异的费城（Ph）易位。荧光原位杂交（FISH）分析显示在22q11位点存在BCR-ABL重排的扩增。据我们所知，这是首次在ALL的衍生22号染色体上观察到重复的BCR-ABL嵌合基因。我们的观察结果支持通过FISH分析在单细胞水平检测变异Ph染色体的可能性。
