体外暴露于低浓度二氧化氮的大鼠肺泡巨噬细胞中醛类物质的释放。

Release of aldehydes from rat alveolar macrophages exposed in vitro to low concentrations of nitrogen dioxide.

作者信息

Robison T W, Forman H J, Thomas M J

机构信息

Department of Molecular Pharmacology and Toxicology, University of Southern California, Los Angeles 90033, USA.

出版信息

Biochim Biophys Acta. 1995 Jun 6;1256(3):334-40. doi: 10.1016/0005-2760(95)00041-a.

DOI:10.1016/0005-2760(95)00041-a

PMID:7786896

Abstract

This study demonstrated that aldehydes are released into the extracellular medium when alveolar macrophages (AM) are exposed to nitrogen dioxide (NO2) at concentrations that impair cell function but do not cause cell death. Butanal, glycolaldehyde, 4-hydroxynonenal, pentanal, pentenal, and hexanal were found. Dinitrophenylhydrazine (DNP) derivitization, thin layer chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry were used to identify the products. Some of the aldehydes have potential toxicity and may be responsible, in part, for altered AM function observed following NO2 exposure.

摘要

本研究表明，当肺泡巨噬细胞（AM）暴露于二氧化氮（NO₂）时，醛类会释放到细胞外介质中，该浓度的二氧化氮会损害细胞功能但不会导致细胞死亡。研究发现了丁醛、乙醇醛、4-羟基壬烯醛、戊醛、戊烯醛和己醛。使用2,4-二硝基苯肼（DNP）衍生化、薄层色谱法、高效液相色谱法和气相色谱-质谱联用技术来鉴定这些产物。其中一些醛类具有潜在毒性，可能部分导致了二氧化氮暴露后观察到的肺泡巨噬细胞功能改变。