Yun B S, Seto H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Biosci Biotechnol Biochem. 1995 May;59(5):876-80. doi: 10.1271/bbb.59.876.
Our continued screening to find tipA promoter-inducing substances resulted in the isolation of promoinducin from a mycelial extract of Streptomyces sp. SF2741. Based on various 1D- and 2D-NMR studies, including field gradient (FG)-COSY, HSQC, FG-HMBC, phase-sensitive 13C-decoupled HMBC and NOESY experiments, promoinducin's structure was established to be a thiopeptide composed of threonine, some unusual amino acids masked at their carboxyl groups by thiazole or methyloxazole rings, sulfomycinamate and five dehydroalanine residues. Promoinducin induced the tipA promoter at 40 ng/ml, and also exhibited strong antibacterial activity against some Gram-positive bacteria.
我们持续进行筛选以寻找诱导tipA启动子的物质，结果从链霉菌属SF2741的菌丝体提取物中分离出了启动子诱导素。基于各种一维和二维核磁共振研究，包括场梯度（FG）-COSY、HSQC、FG-HMBC、相敏13C去偶HMBC和NOESY实验，确定启动子诱导素的结构为一种硫肽，由苏氨酸、一些羧基被噻唑或甲基恶唑环掩盖的不寻常氨基酸、磺胺霉素酸酯和五个脱氢丙氨酸残基组成。启动子诱导素在浓度为40 ng/ml时可诱导tipA启动子，并且对一些革兰氏阳性菌也表现出很强的抗菌活性。
