Rivière M E, Mysiakine E, Barot R, Nobrega T, Fresneau C, Tlaskalova-Hogenova H, Dumitrescu M, Arrio B
CNRS, URA 1116, Université Paris-Sud, Orsay, France.
Cell Mol Biol (Noisy-le-grand). 1995 Mar;41(2):289-96.
A comparative study of Nocardia water soluble mitogen (NWSM) action on membrane potential and proliferation rate of murine peritoneal lymphocytes was performed at various incubation times. The membrane surface charge was evaluated by laser doppler velocimetry (LDV) through the measurement of the cell electrophoretic mobility at different pH values (from pH 5 to 9). We demonstrated that NWSM treatment decreases the lymphocyte membrane potential. This variation reached a maximal level after 24 hrs. at pH 7 and remained unchanged during the 72 hrs. observation. A significant stimulation of lymphocyte proliferation was noted after a 24 hrs. incubation. However, the highest rate of [3H]-thymidine incorporation was observed at 48 hrs. with a subsequent decrease at 72 hrs. On the basis of these data, it is suggested, that membrane potential changes may represent an early important step in the mechanism of lymphocyte activation by NWSM, as it has been shown for some mitogenic compounds.
在不同孵育时间对诺卡氏菌水溶性促细胞分裂剂（NWSM）对小鼠腹膜淋巴细胞膜电位和增殖率的作用进行了比较研究。通过激光多普勒测速仪（LDV）在不同pH值（从pH 5至9）下测量细胞电泳迁移率来评估膜表面电荷。我们证明NWSM处理会降低淋巴细胞膜电位。这种变化在24小时后于pH 7时达到最大水平，并在72小时观察期内保持不变。孵育24小时后观察到淋巴细胞增殖有显著刺激。然而，[3H] - 胸苷掺入率在48小时时最高，随后在72小时时下降。基于这些数据，有人提出，膜电位变化可能是NWSM激活淋巴细胞机制中的一个早期重要步骤，正如一些促细胞分裂化合物所显示的那样。
