Miyazaki H, Kikuchi A, Koyama Y, Okano T, Sakurai Y, Kataoka K
Department of Materials Science, Science University of Tokyo, Chiba, Japan.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):829-36. doi: 10.1006/bbrc.1993.2120.
A novel water-soluble polymer (poly(AAm-co-PBA)) was synthesized by radical copolymerization of 3-acrylamidophenylboronic acid with acrylamide and evaluated as synthetic mitogen of lymphocytes. Boronic acid was introduced in the polymer as recognition site of sugar residues existing on the plasma membrane surface of lymphocyte, since it makes a covalent bonding with polyol compounds including sugars. Obvious increase in [3H]thymidine incorporation of lymphocytes was observed when murine spleen lymphocytes were cultured with poly(AAm-co-PBA), while little proliferative response of lymphocytes was observed in the presence of the homopolymer of acrylamide, the polymer without boronic acid moiety. This suggests that proliferation of lymphocytes was induced through the binding of phenylboronic acid moiety in poly(AAm-co-PBA) with glycoproteins existing on the plasma membrane surface of lymphocytes. Further, poly(AAm-co-PBA) was found to induce even the proliferation of B cell-depleted population of lymphocytes, suggesting T cell stimulation by this boronate-containing polymer.
通过3-丙烯酰胺基苯硼酸与丙烯酰胺的自由基共聚反应合成了一种新型水溶性聚合物（聚（丙烯酰胺-co-苯硼酸）），并将其作为淋巴细胞的合成促有丝分裂原进行了评估。硼酸作为淋巴细胞质膜表面存在的糖残基的识别位点被引入聚合物中，因为它能与包括糖在内的多元醇化合物形成共价键。当用聚（丙烯酰胺-co-苯硼酸）培养小鼠脾淋巴细胞时，观察到淋巴细胞[3H]胸腺嘧啶核苷掺入量明显增加，而在存在丙烯酰胺均聚物（不含硼酸部分的聚合物）的情况下，淋巴细胞几乎没有增殖反应。这表明淋巴细胞的增殖是通过聚（丙烯酰胺-co-苯硼酸）中的苯硼酸部分与淋巴细胞质膜表面存在的糖蛋白结合而诱导的。此外，发现聚（丙烯酰胺-co-苯硼酸）甚至能诱导B细胞耗竭的淋巴细胞群体增殖，表明这种含硼酸盐的聚合物对T细胞有刺激作用。
