Henke W, Jung K, Polster F
Universitätsklinikum Charité, Forschungsabteilung der Urologischen Klinik, Humboldt Universität zu Berlin, F.R.Germany.
Cell Mol Biol (Noisy-le-grand). 1995 Mar;41(2):319-26.
The mitochondria isolated from cortex and medulla of rat kidneys were examined after storage in either Euro-Collins solution, in Bretschneider's histidine-tryptophan-ketoglutarat solution or in Belzer's University of Wisconsin solution at 25 degrees C or at 4 degrees C for a maximum of 4 to 24 hrs. Independently to the preservation of the solution used, the storage of the kidneys led to a decrease in state 3 respiration and uncoupled respiration as well as to an increase in the rate of state 4 respiration. The decrease in state 3 respiration ran parallelly to a decrease in adenine nucleotides. For the homogeneous protection of the mitochondria as well from the cortex as from the medulla, the Bretschneider's solution had the best preservative effect at a storage temperature of 25 degrees C as shown by the measured mitochondrial parameters. At 4 degrees C storage temperature, the differences in action with the various examined solutions were markedly smaller. However, compared to the other two solutions, the Euro-Collins solution showed a more favourable protective effect on cortical and medullary mitochondria.
将大鼠肾脏皮质和髓质分离出的线粒体，分别置于欧林柯林斯溶液、布雷施奈德氏组氨酸 - 色氨酸 - 酮戊二酸溶液或贝尔泽氏威斯康星大学溶液中，于25℃或4℃下储存最多4至24小时后进行检查。与所使用溶液的保存情况无关，肾脏的储存导致状态3呼吸和非偶联呼吸减少，以及状态4呼吸速率增加。状态3呼吸的减少与腺嘌呤核苷酸的减少平行。如所测线粒体参数所示，对于来自皮质和髓质的线粒体的均匀保护，布雷施奈德氏溶液在25℃储存温度下具有最佳的保存效果。在4℃储存温度下，各种受试溶液的作用差异明显较小。然而，与其他两种溶液相比，欧林柯林斯溶液对皮质和髓质线粒体显示出更有利的保护作用。
