Ribeiro C A, Falkenstein D, Figueiredo J F
Department of Physiology and Biophysics, Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.
Cryobiology. 1994 Jun;31(3):272-8. doi: 10.1006/cryo.1994.1033.
The extent of nephron injury after long-term preservation is unknown. An experimental protocol was designed to study the effects of 1 and 24 h of cold storage in preservation solution on renal slices of rabbit cortical collecting duct (CCD) isolated and perfused in vitro and evaluated by water hydraulic conductivity. The preservation solutions were Ringer-bicarbonate, Collins, Euro-Collins, and University of Wisconsin (UW). Under these experimental preservation conditions, the following results were observed: after 1 h of preservation, the CCDs obtained from slices incubated in Ringer-bicarbonate, Collins, and Euro-Collins solutions at 4 degrees C presented a high basal hydraulic conductivity compared to the control group and responded well to arginine-vasopressin (AVP) water permeability stimulation. The CCDs derived from renal slices stored for 24 h in Ringer-bicarbonate solution at 4 degrees C showed no function, while those preserved in Collins and Euro-Collins solutions showed a lack of responsiveness to AVP water permeability stimulation. The CCDs preserved in UW solution showed better values than those preserved in the other solutions, although there was a decrease in hydraulic conductivity after 24 h of preservation. These data show that long-term cold preservation with flushed solutions impairs the cortical collecting duct response to AVP.
长期保存后肾单位损伤的程度尚不清楚。设计了一项实验方案，以研究在保存溶液中1小时和24小时的冷藏对体外分离并灌注的兔皮质集合管（CCD）肾切片的影响，并通过水导性进行评估。保存溶液为林格氏碳酸氢盐溶液、柯林斯溶液、欧洲柯林斯溶液和威斯康星大学（UW）溶液。在这些实验保存条件下，观察到以下结果：保存1小时后，在4℃下于林格氏碳酸氢盐溶液、柯林斯溶液和欧洲柯林斯溶液中孵育的切片所获得的CCD与对照组相比呈现出较高的基础水导性，并且对精氨酸加压素（AVP）的水通透性刺激反应良好。在4℃下于林格氏碳酸氢盐溶液中储存24小时的肾切片所衍生的CCD无功能，而保存在柯林斯溶液和欧洲柯林斯溶液中的CCD对AVP水通透性刺激缺乏反应。保存在UW溶液中的CCD显示出比保存在其他溶液中的更好的值，尽管在保存24小时后水导性有所下降。这些数据表明，用冲洗溶液进行长期冷藏会损害皮质集合管对AVP的反应。
