Watanabe Y, Mori S, Fujiyama S, Sato T, Mori M
Third Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Enzyme Protein. 1994;48(1):18-26. doi: 10.1159/000474965.
We developed a sensitive enzyme-linked immunosorbent assay (ELISA) for serum ornithine carbamoyltransferase (OCT) protein, and examined serum OCT concentrations in patients with various liver diseases. OCT concentrations were markedly elevated in cases of hepatic encephalopathy, 'acute on chronic', and those with the acute phase of acute hepatitis, moderately in chronic hepatitis, liver cirrhosis, hepatocellular carcinoma, primary biliary cirrhosis, and slightly in those with a fatty liver. High percentages (92-98%) of patients with chronic hepatitis, liver cirrhosis and hepatocellular carcinoma had higher than normal concentrations of serum OCT protein. There was a close correlation with aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and moderate correlations with those of mitochondrial AST, glutamate dehydrogenase and gamma-glutamyltranspeptidase. The OCT/ALT ratio was higher in patients with liver cirrhosis than in those with chronic hepatitis (p < 0.001), and was still higher in cases of hepatocellular carcinoma (p < 0.05). In 2 patients with 'acute on chronic' disease, OCT concentrations decreased similarly with or more rapidly than AST or ALT activities after admission. In 2 patients with hepatic encephalopathy, the OCT concentrations changed similarly with AST and ALT activities. This OCT ELISA system will aid in diagnosing various liver diseases and in the follow-up of the patients, and the OCT/ALT ratio may serve for a differential diagnosis of liver diseases.
我们开发了一种用于检测血清鸟氨酸氨甲酰基转移酶（OCT）蛋白的灵敏酶联免疫吸附测定法（ELISA），并检测了各种肝病患者的血清OCT浓度。在肝性脑病、“慢性肝急性发作”以及急性肝炎急性期患者中，OCT浓度显著升高；在慢性肝炎、肝硬化、肝细胞癌、原发性胆汁性肝硬化患者中，OCT浓度中度升高；在脂肪肝患者中，OCT浓度轻度升高。慢性肝炎、肝硬化和肝细胞癌患者中，血清OCT蛋白浓度高于正常水平的比例较高（92 - 98%）。血清OCT浓度与天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）活性密切相关，与线粒体AST、谷氨酸脱氢酶和γ-谷氨酰转肽酶活性中度相关。肝硬化患者的OCT/ALT比值高于慢性肝炎患者（p < 0.001），肝细胞癌患者的该比值更高（p < 0.05）。在2例“慢性肝急性发作”患者中，入院后OCT浓度下降情况与AST或ALT活性相似或更快。在2例肝性脑病患者中，OCT浓度变化与AST和ALT活性相似。这种OCT ELISA系统将有助于各种肝病的诊断和患者的随访，OCT/ALT比值可能有助于肝病的鉴别诊断。
