血清鸟氨酸氨基甲酰转移酶的灵敏酶联免疫吸附测定法。

A sensitive enzyme-linked immunosorbent assay of serum ornithine carbamoyltransferase.

作者信息

Watanabe Y, Mori S, Ozaki M, Fujiyama S, Sato T, Mori M

机构信息

Third Department of Internal Medicine, Kumamoto University School of Medicine, Japan.

出版信息

Enzyme Protein. 1994;48(1):10-7. doi: 10.1159/000474964.

DOI:10.1159/000474964

PMID:7787966

Abstract

Ornithine carbamoyltransferase (OCT), a urea cycle enzyme, is located almost exclusively in liver mitochondria. We designed a sensitive enzyme-linked immunosorbent assay (ELISA) of serum OCT protein using an antibody against purified bovine enzyme. OCT protein measured using this method showed a good correlation with OCT activity (r = 0.961), and was much higher in patients with liver diseases than in the controls. Measurements of serum OCT protein in 442 healthy blood donors gave the upper limit of normal range of 23 ng Eq/ml (equivalent to the bovine enzyme) for males, 8 ng Eq/ml for females, and 16 ng Eq/ml for males plus females. The values differ significantly between the sexes and depending on ages. This ELISA system is expected to be used as a pertinent liver function test.

摘要

鸟氨酸氨甲酰基转移酶（OCT）是一种尿素循环酶，几乎仅存在于肝线粒体中。我们使用针对纯化牛酶的抗体设计了一种灵敏的血清OCT蛋白酶联免疫吸附测定法（ELISA）。用该方法测定的OCT蛋白与OCT活性显示出良好的相关性（r = 0.961），并且在肝病患者中比对照组高得多。对442名健康献血者的血清OCT蛋白进行测量，结果显示男性正常范围上限为23 ng Eq/ml（相当于牛酶），女性为8 ng Eq/ml，男性加女性为16 ng Eq/ml。这些值在性别之间以及随年龄不同而有显著差异。该ELISA系统有望用作相关的肝功能检测。