通过直接测定大鼠肝线粒体中NADH-辅酶Q还原酶对复合物I活性的评估不足。
Underevaluation of complex I activity by the direct assay of NADH-coenzyme Q reductase in rat liver mitochondria.
作者信息
Lenaz G, Fato R, Genova M L, Formiggini G, Parenti Castelli G, Bovina C
机构信息
Dipartimento di Biochimica G. Moruzzi, University of Bologna, Italy.
出版信息
FEBS Lett. 1995 Jun 12;366(2-3):119-21. doi: 10.1016/0014-5793(95)00508-7.
We have shown that the rate of NADH-coenzyme Q reductase in rat liver mitochondria, assayed using the decyl-ubiquinone analog DB, is underevaluated, probably as a result of its low water solubility. In view of drawbacks encountered using other more soluble acceptors in this system, we demonstrate that the most reliable assay of the physiological rate of CoQ reduction by Complex I is the indirect calculation from the total rate of NADH oxidation and the rate of ubiquinol oxidation, using the pool equation of Kröger and Klingenberg [(1973) Eur. J. Biochem. 34, 358-368].
我们已经表明，使用癸基泛醌类似物DB测定的大鼠肝脏线粒体中NADH-辅酶Q还原酶的速率被低估了，这可能是由于其低水溶性所致。鉴于在该系统中使用其他更易溶的受体时遇到的缺点，我们证明，通过复合物I还原辅酶Q的生理速率的最可靠测定方法是根据NADH氧化总速率和泛醇氧化速率，使用Kröger和Klingenberg的池方程[(1973)欧洲生物化学杂志34, 358 - 368]进行间接计算。
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