Abdel-Razzak Z, Corcos L, Fautrel A, Guillouzo A
INSERM U49, Hôpital de Pontchaillou, Rennes, France.
FEBS Lett. 1995 Jun 12;366(2-3):159-64. doi: 10.1016/0014-5793(95)00513-9.
We have investigated the effects of interleukin (IL)-1 beta and IL6 on expression and phenobarbital (PB) induction of ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-deethylase (PROD) activities, as well as on mRNA levels of cytochromes P450 (CYP) 1A, 2B, 2C, 2E and 3A, in rat hepatocytes in primary culture. IL6 slightly antagonized PB-induced PROD activity. Strikingly, IL1 beta strongly inhibited basal EROD and PROD activities, and fully blocked their induction by PB in a dose-dependent fashion. Furthermore IL1 beta completely suppressed PB induction of all CYP mRNAs analyzed. Our results demonstrate that IL1 beta can suppress basal CYP activities, as well as PB-inducible expression of five CYP mRNAs in rat hepatocytes in primary culture.
我们研究了白细胞介素（IL）-1β和IL-6对原代培养大鼠肝细胞中乙氧基异吩恶唑酮O-脱乙基酶（EROD）和戊氧基异吩恶唑酮O-脱乙基酶（PROD）活性的表达及苯巴比妥（PB）诱导作用的影响，以及对细胞色素P450（CYP）1A、2B、2C、2E和3A mRNA水平的影响。IL-6轻微拮抗PB诱导的PROD活性。令人惊讶的是，IL-1β强烈抑制基础EROD和PROD活性，并以剂量依赖方式完全阻断PB对它们的诱导作用。此外，IL-1β完全抑制PB对所有分析的CYP mRNA的诱导作用。我们的结果表明，IL-1β可抑制原代培养大鼠肝细胞中的基础CYP活性以及PB诱导的5种CYP mRNA的表达。
