Chakraborti P, Mukherjee D
Department of Zoology, University of Kalyani, West Bengal, India.
Gen Comp Endocrinol. 1995 Mar;97(3):320-6. doi: 10.1006/gcen.1995.1032.
The effects of prolactin (PRL) from both mammalian and piscine sources on plasma calcium levels in common carp, Cyprinus carpio, were investigated. Injections of ovine prolactin (oPRL) or homologous fish pituitary extract or partially purified prolactin of murrel, Channa punctatus pituitary (mPRL), caused significant increases in total and ultrafiltrable plasma calcium. Larger hypercalcemic responses were observed in fish kept in high-calcium fresh water and normal tap water than in fish in low-calcium fresh water. Injections of oPRL caused dose-dependent increases in plasma calcium level. The highest dose (1 mg/kg body wt) of oPRL had a reduced hypercalcemic effect. Administration of EGTA (200 microM/kg body wt) reduced the stimulatory effects of daily injections of oPRL or mPRL on plasma calcium in freshwater common carp under laboratory conditions. These results indicate that PRL may be involved in regulating plasma calcium levels in fish.
研究了来自哺乳动物和鱼类的催乳素（PRL）对鲤鱼（Cyprinus carpio）血浆钙水平的影响。注射绵羊催乳素（oPRL）、同源鱼垂体提取物或斑点叉尾鮰（Channa punctatus）垂体部分纯化的催乳素（mPRL），会使血浆总钙和超滤性钙显著增加。与饲养在低钙淡水中的鱼相比，饲养在高钙淡水和普通自来水中的鱼出现了更大的高钙血症反应。注射oPRL会导致血浆钙水平呈剂量依赖性增加。oPRL的最高剂量（1毫克/千克体重）具有降低的高钙血症效应。在实验室条件下，给予乙二醇双四乙酸（EGTA，200微摩尔/千克体重）可降低每日注射oPRL或mPRL对淡水鲤鱼血浆钙的刺激作用。这些结果表明，PRL可能参与调节鱼类的血浆钙水平。
