Van de Casteele M, Legrain C, Wilquet V, Glansdorff N
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium.
Gene. 1995 May 26;158(1):101-5. doi: 10.1016/0378-1119(95)00090-s.
The structural gene (dyrA) encoding dihydrofolate reductase (DHFR) of Thermotoga maritima has been cloned, sequenced and expressed in Escherichia coli. The dyrA gene, located immediately upstream from the gene encoding aspartate carbamoyltransferase (pyrB), encodes a highly thermostable enzyme with a distinct thermophilic activity profile. Important structural features are conserved among all bacterial DHFR, yet the DHFR of T. maritima appears unique in a number of insertions and deletions, some of which are reminiscent of eukaryotic DHFR.
嗜热栖热菌二氢叶酸还原酶（DHFR）的结构基因（dyrA）已被克隆、测序并在大肠杆菌中表达。dyrA基因位于天冬氨酸氨甲酰转移酶（pyrB）编码基因的紧邻上游，编码一种具有独特嗜热活性谱的高度耐热酶。所有细菌DHFR中都保守着重要的结构特征，但嗜热栖热菌的DHFR在一些插入和缺失方面显得独特，其中一些让人联想到真核生物的DHFR。
