一种与源自ColE1的质粒兼容的阿拉伯糖诱导型表达载体pAR3。
An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids.
作者信息
Pérez-Pérez J, Gutiérrez J
机构信息
Departamento de Investigación, Pharmacia Antibióticos Farma S.A., Madrid, Spain.
出版信息
Gene. 1995 May 26;158(1):141-2. doi: 10.1016/0378-1119(95)00127-r.
Arabinose-inducible genetic elements from the Salmonella typhimurium arabinose operon were inserted into pACYC184. The resultant plasmid, pAR3, is compatible with ColE1-derived plasmids and allows efficient expression of recombinant (re) genes upon induction with arabinose. These features make it convenient for use in combination with standard gene expression vectors for the independently controlled production of two or more re-polypeptides in Escherichia coli.
来自鼠伤寒沙门氏菌阿拉伯糖操纵子的阿拉伯糖诱导型遗传元件被插入到pACYC184中。所得质粒pAR3与ColE1衍生质粒兼容，并在阿拉伯糖诱导时允许重组（re）基因高效表达。这些特性使其便于与标准基因表达载体结合使用，以便在大肠杆菌中独立控制生产两种或更多种重组多肽。
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