Guan X Y, Meltzer P S, Burgess A C, Trent J M
Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892, USA.
Hum Genet. 1995 Jun;95(6):637-40. doi: 10.1007/BF00209479.
Human chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique "bar codes" and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.
人类6号染色体已通过染色体显微切割被细分为14个独特区域。显微切割后，使用通用引物对切割后的DNA进行聚合酶链反应（PCR）扩增，以生成亚区域特异性探针，这些探针可完全覆盖6号染色体。通过使用与G带正常中期染色体杂交的生物素标记的切割DNA，经荧光原位杂交（FISH）已将所有16次显微切割区域定位到6号染色体上。这些探针可用作区域特异性涂料，以生成独特的“条形码”，并用于分析涉及6号染色体的染色体改变，而这些改变通过传统的带型分析无法识别。
