Polakiewicz R D, Munroe D J, Sait S N, Tycowski K T, Nowak N J, Shows T B, Housman D E, Page D C
Howard Hughes Research Laboratories, Whitehead Institute, Cambridge, Massachusetts 02142, USA.
Genomics. 1995 Jan 20;25(2):577-80. doi: 10.1016/0888-7543(95)80063-r.
The mammalian ribosome is a massive structure composed of 4 RNA species and about 80 different proteins. One of these ribosomal proteins, S3, appears to function not only in translation but also as an endonuclease in repair of UV-induced DNA damage. Moreover, the first intron of human RPS3 transcripts is processed to generate U15A, a small nucleolar RNA. We localized the nested RPS3/U15A genes to the immediate vicinity of D11S356 and D11S533 on human chromosome 11q13.3-q13.5 using a combination of somatic cell hybrid analysis, fluorescence in situ hybridization, and YAC/STS content mapping. These findings add to the evidence that genes encoding ribosomal proteins are scattered about the human genome.
哺乳动物核糖体是一个由4种RNA和大约80种不同蛋白质组成的庞大结构。其中一种核糖体蛋白S3似乎不仅在翻译过程中发挥作用，还作为一种核酸内切酶参与紫外线诱导的DNA损伤修复。此外，人类RPS3转录本的第一个内含子经过加工后产生小核仁RNA U15A。我们通过体细胞杂交分析、荧光原位杂交和YAC/STS含量作图相结合的方法，将嵌套的RPS3/U15A基因定位到人类染色体11q13.3 - q13.5上D11S356和D11S533的紧邻区域。这些发现进一步证明了编码核糖体蛋白的基因分散在人类基因组中。
