Redpath J L, Hyden E C, Sun C
Department of Radiation Oncology, University of California Irvine 92717, USA.
Int J Radiat Oncol Biol Phys. 1995 Jun 15;32(3):681-5. doi: 10.1016/0360-3016(94)00634-W.
To determine if fractionated radiation treatment can alter cisplatinum sensitivity of a human laryngeal squamous carcinoma cell line.
Human squamous carcinoma cells, both previously untreated, as well as survivors of fractionated radiation therapy, were tested in vitro for their sensitivity to gamma radiation and cisplatinum. Fractionated gamma radiation was delivered in 14 or 10 daily fractions of 2 Gy. The cell line, cSCC-20, was derived from an untreated primary human laryngeal carcinoma.
The human laryngeal squamous cell carcinoma cell line, cSCC-20, was demonstrated to have heterogeneous subpopulations with respect to cisplatinum sensitivity. No variation in radiation sensitivity was seen among subpopulations of varying cisplatinum sensitivity. The cells were relatively radioresistant (Do = 2.5 Gy). Fractionated radiation treatments of the parent cell line (14 fractions, 14 days, 2 Gy/fraction) or a cisplatinum sensitive subline (10 fractions, 12 days, 2 Gy/fraction) induced cisplatinum sensitivity (factor of 1.3 to 1.4) in the surviving cells.
Fractionated radiation treatment of human squamous carcinoma cells in vitro induced sensitivity to cisplatinum without concomitant alteration in radiation sensitivity.
确定分次放射治疗是否能改变人喉鳞状癌细胞系对顺铂的敏感性。
对未经治疗的人鳞状癌细胞以及分次放射治疗后的存活细胞进行体外测试，检测其对γ射线和顺铂的敏感性。分次γ射线以每天2 Gy的剂量分14次或10次给予。细胞系cSCC - 20源自未经治疗的原发性人喉癌。
人喉鳞状癌细胞系cSCC - 20在顺铂敏感性方面表现出异质性亚群。不同顺铂敏感性亚群之间未观察到放射敏感性的差异。这些细胞相对放射抗拒（Do = 2.5 Gy）。对亲代细胞系（14次分割，14天，每次分割2 Gy）或顺铂敏感亚系（10次分割，12天，每次分割2 Gy）进行分次放射治疗，可诱导存活细胞对顺铂敏感（敏感性提高1.3至1.4倍）。
体外对人鳞状癌细胞进行分次放射治疗可诱导其对顺铂敏感，而放射敏感性无相应改变。
