Rahner-Welsch S, Vogel J, Kuschinsky W
Department of Physiology, University of Heidelberg, Germany.
J Cereb Blood Flow Metab. 1995 Jul;15(4):681-6. doi: 10.1038/jcbfm.1995.84.
The association of glucose transporters (GLUT1) and brain capillaries was tested in different brain structures of rats by a direct comparison of the topologies of capillaries and GLUT1 in identical brain sections. Antibody staining of capillaries (fibronectin) and GLUT1 were made visible by fluorescence microscopy. The results showed differences between brain structures containing a tight and a leaky blood-brain barrier. All capillaries of brain structures with a tight blood-brain barrier showed congruent staining of GLUT1 and capillary morphology. The circumventricular organs that are known to have leaky barrier capillaries were stained by fibronectin antibodies but not by GLUT1 antibodies. Ependymal cells showed moderate staining by GLUT1 antibodies both in areas with tight and leaky barriers. The subcommissural organ appeared to be unique showing neither capillary nor GLUT1 stain. It is concluded that glucose transporters (GLUT1) exist in all brain capillaries of blood-brain barrier structures, whereas they are absent in leaky barrier structures. Moderate amounts of glucose transporter (GLUT1) can also be detected in ependymal cells.
通过直接比较相同脑切片中毛细血管和葡萄糖转运蛋白1（GLUT1）的拓扑结构，在大鼠的不同脑结构中检测了葡萄糖转运蛋白（GLUT1）与脑毛细血管的关联。通过荧光显微镜观察毛细血管（纤连蛋白）和GLUT1的抗体染色情况。结果显示，具有紧密型和渗漏型血脑屏障的脑结构之间存在差异。具有紧密血脑屏障的脑结构中的所有毛细血管，其GLUT1染色与毛细血管形态一致。已知具有渗漏型屏障毛细血管的室周器官，用纤连蛋白抗体染色，但不用GLUT1抗体染色。室管膜细胞在具有紧密和渗漏屏障的区域均显示出GLUT1抗体的中度染色。连合下器官似乎很独特，既没有毛细血管染色也没有GLUT1染色。得出的结论是，葡萄糖转运蛋白（GLUT1）存在于血脑屏障结构的所有脑毛细血管中，而在渗漏型屏障结构中不存在。在室管膜细胞中也可检测到适量的葡萄糖转运蛋白（GLUT1）。
