Wang Y H, Bosy T Z, Yasuda R P, Grayson D R, Vicini S, Pizzorusso T, Wolfe B B
Department of Pharmacology, Georgetown University School of Medicine, Washington, DC 20007, USA.
J Neurochem. 1995 Jul;65(1):176-83. doi: 10.1046/j.1471-4159.1995.65010176.x.
Selective antisera for NMDA receptor subunits NR2A and NR2B have been developed. Each antiserum identifies a single band on an immunoblot at approximately 175 kDa that appears to be the appropriate subunit of the NMDA receptor. Using these antisera the relative densities of the subunits in eight areas of adult rat brain have been determined. The NR2A subunit was found to be at its highest level in hippocampus and cerebral cortex, to be at intermediate levels in striatum, olfactory tubercle, mid-brain, olfactory bulb, and cerebellum, and to be at lowest levels in the pons-medulla. The NR2B subunit was found to be expressed at its highest levels in the olfactory tubercle, hippocampus, olfactory bulb, and cerebral cortex. Intermediate levels were expressed in striatum and mid-brain, and low levels were detected in the pons-medulla. No signal for NR2B was found in the cerebellum. These regional distributions were compared with that for [3H]MK-801 binding sites. It was found that although the distribution of the NR2A subunit corresponds well with radioligand binding, the distribution of the NR2B subunit does not. The ontogenic profiles of NR2A and NR2B subunits in the rat cerebellum were also determined. Just following birth [postnatal day (P) 2] NR2A subunits are undetectable, whereas NR2B subunits are expressed at amounts easily measurable. Beginning at about P12 the levels of NR2A rise rapidly to reach adult levels by P22. At the same time (P12), levels of NR2B protein begin to decline rapidly to reach undetectable levels by 22 days after birth.(ABSTRACT TRUNCATED AT 250 WORDS)
已开发出针对NMDA受体亚基NR2A和NR2B的选择性抗血清。每种抗血清在免疫印迹上可识别出一条约175 kDa的单带，该条带似乎是NMDA受体的相应亚基。利用这些抗血清，已测定了成年大鼠脑八个区域中亚基的相对密度。发现NR2A亚基在海马体和大脑皮层中含量最高，在纹状体、嗅结节、中脑、嗅球和小脑中含量中等，而在脑桥-延髓中含量最低。发现NR2B亚基在嗅结节、海马体、嗅球和大脑皮层中表达水平最高。在纹状体和中脑中表达水平中等，在脑桥-延髓中检测到低水平表达。在小脑中未发现NR2B的信号。将这些区域分布与[3H]MK-801结合位点的分布进行了比较。发现尽管NR2A亚基的分布与放射性配体结合情况吻合良好，但NR2B亚基的分布并非如此。还测定了大鼠小脑中NR2A和NR2B亚基的个体发育情况。刚出生后（出生后第2天），NR2A亚基无法检测到，而NR2B亚基以易于测量的量表达。从大约出生后第12天开始，NR2A的水平迅速上升，到出生后第22天达到成年水平。同时（出生后第12天），NR2B蛋白水平开始迅速下降，到出生后22天降至无法检测到的水平。（摘要截短于250字）
