Takahashi S, Cook M, Jehle J, Kennedy C, Sokoloff L
Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892, USA.
J Neurochem. 1995 Jul;65(1):414-9. doi: 10.1046/j.1471-4159.1995.65010414.x.
The effects of chronic treatment with NG-nitro-L-arginine methyl ester, a potent inhibitor of nitric oxide synthase activity, on local cerebral glucose utilization were examined in conscious rats. Intraperitoneal injections of 50 mg/kg of the nitroarginine twice daily for 4 days have been found to result in almost complete inhibition of nitric oxide synthase activity in brain. Local cerebral glucose utilization was determined by means of the quantitative autoradiographic [14C]deoxyglucose method in an experimental group (n = 7) of rats that were treated with the nitroarginine according to this schedule and in a normal control group (n = 7) treated similarly with saline. The rats were conscious but partially restrained during the determinations of local cerebral glucose utilization. The nitroarginine treatment raised mean arterial blood pressure statistically significantly to 147 +/- 3 mm Hg (mean +/- SEM) from a level of 120 +/- 5 mm Hg in the saline controls (p < 0.001 by grouped t test), but there were no statistically significant effects on glucose utilization in any of 39 brain structures examined. It is concluded that nitric oxide normally exerts no significant influence on energy metabolism in the rat brain.
在清醒大鼠中，研究了一氧化氮合酶活性的强效抑制剂NG-硝基-L-精氨酸甲酯长期治疗对局部脑葡萄糖利用的影响。已发现，每天两次腹腔注射50mg/kg硝基精氨酸，持续4天，可导致大脑中一氧化氮合酶活性几乎完全受到抑制。采用定量放射自显影[14C]脱氧葡萄糖法，对按照该方案用硝基精氨酸治疗的实验组大鼠（n = 7）和同样用生理盐水治疗的正常对照组大鼠（n = 7）的局部脑葡萄糖利用情况进行了测定。在测定局部脑葡萄糖利用期间，大鼠处于清醒但部分受限的状态。硝基精氨酸治疗使平均动脉血压从生理盐水对照组的120±5mmHg统计学显著升高至147±3mmHg（均值±标准误）（成组t检验，p < 0.001），但在所检查的39个脑结构中的任何一个中，对葡萄糖利用均无统计学显著影响。得出的结论是，一氧化氮通常对大鼠脑内的能量代谢无显著影响。
