1型人类免疫缺陷病毒可通过聚合酶链反应在单核细胞中检测到。
Human immunodeficiency virus type-1 can be detected in monocytes by polymerase chain reaction.
作者信息
Quirós E, García F, Maroto M C, Bernal M C, Cabezas T, Piedrola G
机构信息
Department of Microbiology, Faculty of Medicine, University of Granada, Spain.
出版信息
J Med Microbiol. 1995 Jun;42(6):411-4. doi: 10.1099/00222615-42-6-411.
Lymphocytes and monocytes from 25 patients infected with human immunodeficiency virus type-1 (HIV-1)--13 asymptomatic, seven with the AIDS-related complex (ARC) and five with the acquired immunodeficiency syndrome (AIDS)--were lysed and subjected to PCR with three primer pairs: SK38/SK39 (gag), SK68/SK69 (env) and SK29/SK30 (LTR). Amplified DNA was solution-hybridised with 32P-labelled probes (SK19, SK70 and SK31, respectively) and detected by PAGE-autoradiography. HIV-1 DNA was detected as follows. Asymptomatic patients: monocytes--gag 61.5%, env 100%, LTR 0%; lymphocytes--gag 100%, env 92.3%, LTR 53.84%. ARC patients: monocytes--gag 71.4%, env 57.1%, LTR 0%; lymphocytes--gag 100%, env 71.4%, LTR 71.4%. AIDS patients: monocytes--gag 80.0%, env 100%, LTR 0%; lymphocytes--gag 100%, env 60%, LTR 60%. The presence of HIV-1 DNA was confirmed in the monocyte fraction. In this cell subset, the env gene-directed primers were the most effective for amplification, whereas the LTR gene-directed primers failed to amplify HIV-1 DNA. The different pattern of amplification found in monocytes may suggest that these cells could be infected by a genetic variant of the virus.
对25例感染了1型人类免疫缺陷病毒（HIV-1）的患者的淋巴细胞和单核细胞进行检测——其中13例无症状，7例患有艾滋病相关综合征（ARC），5例患有获得性免疫缺陷综合征（AIDS）——将这些细胞裂解后，使用三对引物进行聚合酶链反应（PCR）：SK38/SK39（gag基因）、SK68/SK69（env基因）和SK29/SK30（长末端重复序列，LTR）。扩增的DNA与32P标记的探针（分别为SK19、SK70和SK31）进行溶液杂交，并通过聚丙烯酰胺凝胶电泳-放射自显影进行检测。HIV-1 DNA的检测结果如下。无症状患者：单核细胞——gag基因61.5%，env基因100%，LTR 0%；淋巴细胞——gag基因100%，env基因92.3%，LTR 53.84%。ARC患者：单核细胞——gag基因71.4%，env基因57.1%，LTR 0%；淋巴细胞——gag基因100%，env基因71.4%，LTR 71.4%。艾滋病患者：单核细胞——gag基因80.0%，env基因100%，LTR 0%；淋巴细胞——gag基因100%，env基因60%，LTR 60%。在单核细胞组分中证实存在HIV-1 DNA。在这个细胞亚群中，env基因定向引物对扩增最为有效，而LTR基因定向引物未能扩增出HIV-1 DNA。在单核细胞中发现的不同扩增模式可能表明这些细胞可能被病毒的基因变异体感染。
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