Döhler J R, Hennig F F, Hughes S P
Abteilung für Unfall-und Wiederherstellungschirurgie, Allgemeines Krankenhaus Altona, Hamburg.
Langenbecks Arch Chir. 1995;380(3):176-83. doi: 10.1007/BF00207726.
In order to study any morphological effects that vasoactive drugs might exert on cortical bone capillaries, Swiss mice received one intravenous bolus injection each of epinephrine, ATP and insulin. In one control group saline solution was injected and another was not treated. All animals were handled in the same way. A piece of the tibia diaphysis was resected and prepared for transmission electron microscopy (TEM). The lumina and the endothelia of capillaries were submitted to computerized morphometry. Some significant changes were noted: epinephrine increases both the width of the lumen and the endothelium. ATP decreases the endothelium. Insulin (hypoglycaemia?) thickens the endothelium. These finding suggest some physiological hypotheses: the epinephrine-induced widening of the lumen and the thickening of the endothelium might reflect a decreased extravasal space and oedema of cortical bone that might cause the diffusion of minerals to take longer. Intracortical perfusion pressure would then decrease and the bone perfusion rate increase. ATP might reduce the transcapillary diffusion time and increase the extravasal space in cortical bone. Apparently there are specific insulin receptors in cortical bone capillaries.
为了研究血管活性药物可能对皮质骨毛细血管产生的任何形态学影响，给瑞士小鼠分别静脉注射肾上腺素、三磷酸腺苷（ATP）和胰岛素。在一个对照组中注射生理盐水，另一个对照组不进行处理。所有动物的处理方式相同。切除一小块胫骨骨干并制备用于透射电子显微镜（TEM）观察。对毛细血管的管腔和内皮进行计算机形态测量。观察到一些显著变化：肾上腺素会增加管腔宽度和内皮厚度。ATP会使内皮变薄。胰岛素（低血糖？）会使内皮增厚。这些发现提示了一些生理学假设：肾上腺素引起的管腔增宽和内皮增厚可能反映皮质骨血管外间隙减小和水肿，这可能导致矿物质扩散时间延长。皮质内灌注压随后会降低，骨灌注率增加。ATP可能会减少跨毛细血管扩散时间并增加皮质骨血管外间隙。显然，皮质骨毛细血管中存在特定的胰岛素受体。
