Hotta S, Kashimura H, Hirai S, Nakahara A, Fukutomi H, Osuga T, Uchiyama Y
Department of Gastroenterology, University of Tsukuba, Ibaraki-Ken, Japan.
Lasers Surg Med. 1995;16(3):262-71. doi: 10.1002/lsm.1900160308.
To further understand the precise process of the tumor cell degeneration after photodynamic therapy (PDT), laser hyperthermic therapy (LH), and combined treatments using an Nd:YAG laser. It is important to examine initial morphological alteration of tumor cells after these treatments.
STUDY DESIGN/MATERIALS AND METHODS: In this study, nude mice bearing HeLa cell tumors were treated with PDT, LH, and combined treatments of the two. Tumor tissues obtained immediately after these treatments were analyzed using electron microscopy and morphometry.
In the combined treatments, which produced more severe effects on tumor cells, morphological features of apoptosis such as cytoplasmic condensation, blebs, and apoptotic bodies appeared in the cells, although the typical alteration in the nuclear chromatin was not seen.
Cytoplasmic alterations may proceed more rapidly than nuclear alterations in the cellular degeneration induced by the single or combined treatments of PDT and LH.
为了进一步了解光动力疗法（PDT）、激光热疗（LH）以及使用钕钇铝石榴石激光的联合治疗后肿瘤细胞变性的精确过程。检查这些治疗后肿瘤细胞的初始形态改变很重要。
研究设计/材料与方法：在本研究中，对荷HeLa细胞肿瘤的裸鼠进行PDT、LH以及两者的联合治疗。使用电子显微镜和形态测量法对这些治疗后立即获取的肿瘤组织进行分析。
在对肿瘤细胞产生更严重影响的联合治疗中，细胞出现了诸如细胞质浓缩、气泡和凋亡小体等凋亡的形态特征，尽管未观察到核染色质的典型改变。
在PDT和LH的单一或联合治疗诱导的细胞变性中，细胞质改变可能比核改变进展得更快。
