Yu D, Liu R, Zhou J, Liu Y
Department of Anatomy, College of Basic Medical Sciences, Tongji Medical University, Wuhan 430030, China.
J Tongji Med Univ. 2000;20(4):268-72. doi: 10.1007/BF02888176.
A computer analysis system, automated imaging cytometry (AIC), flow cytometry (FCM) and EM, were used to study the relationship of apoptosis and the change of DNA in mouse HepA cells subjected to hyperthermia. Our results showed that pyknotic rate in the 44 degrees C group was significantly increased (P < 0.01) when compared with that in the 37 degrees C group, both the diploid rate and aneuploid rate in the 44 degrees C group were significantly decreased (P < 0.01) when compared with that in the 37 degrees C group. The pyknotic rate determined by ALC was in conformity with the apoptotic index (AI) determined by FCM. Under EM, ultrastructures of HapA cells appeared typical apoptosis changes in the 44 degrees C group. We are led to conclude that (1) when the temperature is 42 degrees C or 44 degrees C for 2 h, it did not promote the necrosis of HepA cells; (2) when the temperature is 42 degrees C for 2 h, it did not obviously induce the apoptosis of HepA cells, and the 44 degrees C for 2 h obviously induced the apoptosis of HepA cells; (3) AIC method can be used as an aid for the measurement of apoptotic index. Further study is needed to established it as a novel, simple and convenient method for determining the apoptosis.
采用计算机分析系统、自动成像细胞术（AIC）、流式细胞术（FCM）及电子显微镜（EM），研究热疗后小鼠肝癌细胞凋亡与DNA变化的关系。结果显示，44℃组的核固缩率与37℃组相比显著升高（P<0.01），44℃组的二倍体率和非整倍体率与37℃组相比均显著降低（P<0.01）。AIC测定的核固缩率与FCM测定的凋亡指数（AI）相符。在电子显微镜下，44℃组HapA细胞的超微结构呈现典型的凋亡变化。我们得出以下结论：（1）当温度为42℃或44℃持续2小时，未促进肝癌细胞坏死；（2）当温度为42℃持续2小时，未明显诱导肝癌细胞凋亡，而44℃持续2小时明显诱导肝癌细胞凋亡；（3）AIC方法可辅助测量凋亡指数。需要进一步研究将其确立为一种新颖、简便的凋亡测定方法。
