Kawaguchi N, Fujitani N, Schaper J, Onishi S
School of Allied Health Sciences, Faculty of Medicine, Osaka University, Japan.
Mol Cell Biochem. 1995 Mar 9;144(1):75-9. doi: 10.1007/BF00926743.
Immunocytochemical investigation was performed on the cytoskeletal proteins in cardiac tissue of the cardiomyopathic hamster. Male cardiomyopathic UM-X7.1 hamsters at 180 days of age (n = 8) and age- and sex-matched normal BIO-RB hamsters (n = 8) were used in this study. Immunofluorescence microscopy using monoclonal antibodies against desmin, alpha-actinin, titin, and vincullin was employed. The heart weight to body weight ratio was significantly increased in the heart of cardiomyopathic hamster compared with that of normal hamster. In cardiomyopathic hamster, the left ventricular cavity was markedly dilated. Light microscopically, hypertrophy and atrophy of myocytes and myocardial fibrosis were prominently observed in cardiomyopathic myocardium. Immunocytochemically, desmin, alpha-actinin and titin showed the cross striations along the myofibers in normal myocardium. In contrast, in cardiomyopathic myocardium, desmin was irregularly distributed in myocytes and the amount of desmin was increased. Loss of cross striations of alpha-actinin and titin were frequently observed. Immunofluorescence against vinculin was not significantly altered. We conclude that the alterations of cytoskeletal proteins in myocardial cells may relate to decreased myocardial function in cardiomyopathic hamster failing heart.
对心肌病仓鼠心脏组织中的细胞骨架蛋白进行了免疫细胞化学研究。本研究使用了180日龄的雄性心肌病UM-X7.1仓鼠（n = 8）以及年龄和性别匹配的正常BIO-RB仓鼠（n = 8）。采用针对结蛋白、α-辅肌动蛋白、肌联蛋白和纽蛋白的单克隆抗体进行免疫荧光显微镜检查。与正常仓鼠相比，心肌病仓鼠心脏的心脏重量与体重之比显著增加。在心肌病仓鼠中，左心室腔明显扩张。光镜下，在心肌病心肌中明显观察到心肌细胞肥大和萎缩以及心肌纤维化。免疫细胞化学显示，在正常心肌中，结蛋白、α-辅肌动蛋白和肌联蛋白沿肌纤维显示出横纹。相比之下，在心肌病心肌中，结蛋白在心肌细胞中分布不规则且结蛋白量增加。经常观察到α-辅肌动蛋白和肌联蛋白横纹消失。针对纽蛋白的免疫荧光没有明显改变。我们得出结论，心肌细胞中细胞骨架蛋白的改变可能与心肌病仓鼠衰竭心脏的心肌功能下降有关。
