Fujitani N, Kawaguchi N, Toda S, Matsumura S, Kimura H, Onishi S
Department of Legal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Mol Cell Biochem. 1997 Apr;169(1-2):73-8. doi: 10.1023/a:1006829700747.
An immunocytochemical study was performed to examine the expression of cellular c-myc protein in the heart of 30-, 120- and 180-day-old cardiomyopathic Syrian UM-X7.1 hamsters. The heart of age- and sex-matched BIO-RB hamster was used as normal control. In paraffin sections, an immunostaining for c-myc was markedly increased in cytoplasm of cells from the UM-X7.1 heart as compared with that of the BIO-RB heart which showed a weak staining. However, c-myc was localized in nuclei of cells in frozen sections of the heart. Specific cell types of the heart were differentiated with anti-vimentin, and we found that the increased expression of c-myc was present in nuclei of muscle cells of the UM-X7.1 myocardium. A quantitative study of c-myc-positive nuclei of muscle and nonmuscle cells was carried out by a video micrometer. The mean number of c-myc-positive nuclei of muscle cells was significantly higher in the cardiomyopathic heart than in the control heart from hamsters of all ages studied. These results suggest that the increase of c-myc protein may relate to the pathological state or pathogenesis of the hereditary cardiomyopathy.
进行了一项免疫细胞化学研究，以检测30日龄、120日龄和180日龄患心肌病的叙利亚UM-X7.1仓鼠心脏中细胞c-myc蛋白的表达。将年龄和性别匹配的BIO-RB仓鼠的心脏用作正常对照。在石蜡切片中，与显示弱阳性的BIO-RB心脏相比，UM-X7.1心脏细胞胞质中的c-myc免疫染色明显增加。然而，在心脏冰冻切片中，c-myc定位于细胞核。用抗波形蛋白区分心脏的特定细胞类型，我们发现在UM-X7.1心肌的肌肉细胞核中存在c-myc表达增加。通过视频测微计对肌肉和非肌肉细胞的c-myc阳性细胞核进行了定量研究。在所有研究年龄的仓鼠中，患心肌病心脏的肌肉细胞c-myc阳性细胞核的平均数量显著高于对照心脏。这些结果表明，c-myc蛋白的增加可能与遗传性心肌病的病理状态或发病机制有关。
