Caria H, Chaveca T, Laires A, Rueff J
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Portugal.
Mutat Res. 1995 Jun;343(2-3):85-94. doi: 10.1016/0165-1218(95)90075-6.
Quercetin, a mutagenic flavonoid widely distributed in edible plants, was studied for the induction of micronuclei (MN). We have carried out the MN assay in bone marrow polychromatic erythrocytes in mice, in cytokinesis-blocked human lymphocytes and in cytokinesis-blocked V79 cells. MN assay in vitro was performed in the presence and in the absence of S9. To further extend the study, an antikinetochore antibody (CREST staining) was used to distinguish MN containing whole chromosomes (kinetochore positive) from those containing acentric fragments (kinetochore negative). When tested in vivo quercetin failed to induce micronuclei, a result which is in agreement with other published reports. When tested in vitro in V79 cells quercetin clearly induces micronuclei in the absence of S9 and also in the presence of S9 for the highest dose used. When tested in vitro in human lymphocytes quercetin shows a significant induction of micronuclei in the absence and in the presence of S9. The presence of S9 compared to its absence is not significant for any of the systems used. Both in the presence and absence of S9, quercetin appears to behave as a clastogenic agent in human lymphocytes inducing a significant majority of kinetochore-negative MN.
槲皮素是一种广泛分布于可食用植物中的诱变黄酮类化合物，已对其诱导微核（MN）的情况进行了研究。我们在小鼠骨髓多染性红细胞、胞质分裂阻滞的人淋巴细胞以及胞质分裂阻滞的V79细胞中进行了微核试验。体外微核试验在有和没有S9的情况下进行。为了进一步扩展研究，使用抗动粒抗体（着丝粒蛋白C染色）来区分含有整条染色体的微核（着丝粒阳性）和含有无着丝粒片段的微核（着丝粒阴性）。当在体内进行测试时，槲皮素未能诱导微核，这一结果与其他已发表的报告一致。当在体外对V79细胞进行测试时，槲皮素在没有S9的情况下以及在所使用的最高剂量下有S9存在时均能明显诱导微核。当在体外对人淋巴细胞进行测试时，槲皮素在没有S9和有S9存在的情况下均显示出显著的微核诱导作用。对于所使用任何系统而言，有S9与没有S9相比并无显著差异。无论有无S9，槲皮素在人淋巴细胞中似乎都表现为一种致断裂剂，诱导产生绝大多数着丝粒阴性的微核。
