Cui J, Arita Y, Bystryn J C
Ronald O. Perelman Department of Dermatology, New York University, USA.
Pigment Cell Res. 1995 Feb;8(1):53-9. doi: 10.1111/j.1600-0749.1995.tb00774.x.
Patients with vitiligo have circulating antibodies directed in part to pigment cell antigens with MWs of approximately 90, 75, and 40-45 kDs. These antigens are denominated VIT 90, VIT 75, and VIT 40, respectively. To further characterize these "vitiligo" antigens, we examined their relation to antigens defined by a panel of 25 monoclonal antibodies (moab) to pigment cell antigens. We found by immunoprecipitation and SDS-PAGE analysis of 125I labelled, detergent soluble, human melanocyte macromolecules, that 24 (83%) of 29 patients with vitiligo had antibodies to one or more vitiligo antigens vs. 2 (7%) of 28 control individuals. Seventeen of the 25 moabs did not react with any labelled antigen in the same lysate. Of the remaining eight moabs, only four precipitated an antigen that co-migrated with one of the vitiligo antigens. Moab TA99, HMSA-5, and TMH-1 (all directed to the 75 kD tyrosinase-related protein [TRP1]) co-migrated with VIT 75. Moab W6/32 (directed to class I HLA antigen) co-migrated with VIT 40. Immunodepletion studies with vitiligo antibodies selectively depleted the antigen defined by W6/32 but not the antigen defined by TA99 and HMSA-5, indicating that VIT 75 was not the 75 kD tyrosinase-related protein. The vitiligo antigens were easily labelled by the lactoperoxidase technique but poorly labelled with 35S-methionine, suggesting they are expressed on the cell surface. These studies indicate that VIT 90 and VIT 75 differ from antigens defined by currently available moabs to pigment cell antigens. VIT 40 appears to share a cross-reactive epitope, or be tightly bound to, class I HLA antigen.
白癜风患者体内存在循环抗体，部分抗体针对分子量约为90、75和40 - 45 kD的色素细胞抗原。这些抗原分别命名为VIT 90、VIT 75和VIT 40。为了进一步表征这些“白癜风”抗原，我们研究了它们与一组针对色素细胞抗原的25种单克隆抗体（moab）所定义的抗原之间的关系。通过对125I标记的、去污剂可溶的人黑素细胞大分子进行免疫沉淀和SDS - PAGE分析，我们发现29例白癜风患者中有24例（83%）对一种或多种白癜风抗原有抗体，而28例对照个体中只有2例（7%）有抗体。25种moab中有17种在同一裂解物中不与任何标记抗原发生反应。在其余8种moab中，只有4种沉淀出一种与白癜风抗原之一共迁移的抗原。Moab TA99、HMSA - 5和TMH - 1（均针对75 kD酪氨酸酶相关蛋白[TRP1]）与VIT 75共迁移。Moab W6/32（针对I类HLA抗原）与VIT 40共迁移。用白癜风抗体进行的免疫耗竭研究选择性地耗尽了由W6/32定义的抗原，但没有耗尽由TA99和HMSA - 5定义的抗原，这表明VIT 75不是75 kD酪氨酸酶相关蛋白。白癜风抗原很容易被乳过氧化物酶技术标记，但用35S - 甲硫氨酸标记效果不佳，这表明它们在细胞表面表达。这些研究表明，VIT 90和VIT 75与目前可用的针对色素细胞抗原的moab所定义的抗原不同。VIT 40似乎与I类HLA抗原共享一个交叉反应表位，或与之紧密结合。
