Bartel B, Fink G R
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142, USA.
Science. 1995 Jun 23;268(5218):1745-8. doi: 10.1126/science.7792599.
In plants, the growth regulator indole-3-acetic acid (IAA) is found both free and conjugated to a variety of amino acids, peptides, and carbohydrates. IAA conjugated to leucine has effects in Arabidopsis thaliana similar to those of free IAA. The ilr1 mutant is insensitive to exogenous IAA-Leu and was used to positionally clone the Arabidopsis ILR1 gene. ILR1 encodes a 48-kilodalton protein that cleaves IAA-amino acid conjugates in vitro and is homologous to bacterial amidohydrolase enzymes. DNA sequences similar to that of ILR1 are found in other plant species.
在植物中，生长调节剂吲哚 - 3 - 乙酸（IAA）以游离形式以及与多种氨基酸、肽和碳水化合物结合的形式存在。与亮氨酸结合的IAA在拟南芥中的作用与游离IAA相似。ilr1突变体对外源IAA - 亮氨酸不敏感，被用于通过定位克隆拟南芥ILR1基因。ILR1编码一种48千道尔顿的蛋白质，该蛋白质在体外可切割IAA - 氨基酸共轭物，并且与细菌酰胺水解酶同源。在其他植物物种中也发现了与ILR1相似的DNA序列。
