Sabo V, Bod'a K, Chrappa V
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji.
Vet Med (Praha). 1995 May;40(5):147-50.
An experiment was conducted on Japanese quail and hen hatching eggs that had been incubated, and the embryos were subjected to hypergravitation of 10 G for 10 min on days 1 to 9 of embryonic development. Both the experimental group and the control contained each 20 hatching eggs every day of the experiment (360 eggs in total). A centrifuge of the diameter 1,300 mm was used to create overload (hypergravitation) at the speed of 118 rotations per min. Tab. I shows the layout of the experiment. Embryonic mortality in Japanese quail was investigated during incubation by egg candling on days 1 to 8 and 9 to 14 while suffocated embryos were investigated on days 15 to 17. As for chicks, embryonic mortality was determined by egg candling on days 1 to 8 and 9 to 18, suffocated embryos were determined on days 19 to 21. After incubation was terminated, hatchability in per cent of the fertilized eggs was determined. The results were processed in two stages of development: the first stage days 1 to 5, the second stage days 6 to 9. As can be seen in Fig. 1, hypergravitation did not influence the hatchability of quail eggs in the first stage. But this experimental treatment resulted in a steep fall of hatchability in the second stage of observation in comparison with the control group (the difference is significant P < 0.01). Fig. 2 shows hatchability.(ABSTRACT TRUNCATED AT 250 WORDS)
对已孵化的日本鹌鹑和母鸡种蛋进行了一项实验,在胚胎发育的第1至9天,使胚胎承受10G的超重力10分钟。实验期间,实验组和对照组每天各有20枚种蛋(共360枚蛋)。使用直径1300毫米的离心机,以每分钟118转的速度产生过载(超重力)。表一展示了实验布局。在孵化期间,通过第1至8天以及第9至14天的照蛋来调查日本鹌鹑的胚胎死亡率,而在第15至17天调查窒息胚胎。对于雏鸡,通过第1至8天以及第9至18天的照蛋来确定胚胎死亡率,在第19至21天确定窒息胚胎。孵化结束后,确定受精蛋的孵化率百分比。结果在两个发育阶段进行处理:第一阶段为第1至5天,第二阶段为第6至9天。如图1所示,超重力在第一阶段并未影响鹌鹑蛋的孵化率。但与对照组相比,这种实验处理在第二阶段观察中导致孵化率急剧下降(差异显著,P < 0.01)。图2展示了孵化率。(摘要截选至250字)