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蓝舌病病毒核心颗粒的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of the core particle of bluetongue virus.

作者信息

Burroughs J N, Grimes J M, Mertens P P, Stuart D I

机构信息

Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.

出版信息

Virology. 1995 Jun 20;210(1):217-20. doi: 10.1006/viro.1995.1335.

DOI:10.1006/viro.1995.1335
PMID:7793074
Abstract

Core particles of bluetongue virus serotype 1 (South Africa) have been crystallized. The crystals, which grow up to 0.8 mm in diameter, belong to a primitive orthorhombic space group and have point group symmetry 222. The unit cell dimensions are 754 x 796 x 823 A3 and the crystallographic asymmetric unit contains one-half of a core particle. The best crystals diffract strongly to 4.8 A Bragg spacings, which is the maximum resolution to which we can measure data with the detectors available, suggesting that useful diffraction extends well beyond this. Core particles of serotype 10 have also been crystallized but the crystals have yet to be analyzed by X-ray diffraction.

摘要

蓝舌病病毒血清型1(南非)的核心颗粒已被结晶。这些晶体直径可达0.8毫米,属于原始正交空间群,具有点群对称性222。晶胞尺寸为754×796×823 ų,晶体学不对称单元包含半个核心颗粒。最好的晶体能强烈衍射至4.8 Å的布拉格间距,这是我们使用现有探测器能测量数据的最大分辨率,表明有用的衍射范围远不止于此。血清型10的核心颗粒也已被结晶,但这些晶体尚未通过X射线衍射进行分析。

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