牛心可溶性14 kDaβ-半乳糖苷结合凝集素的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of the soluble 14 kDa beta-galactoside-binding lectin from bovine heart.

作者信息

Bourne Y, Bolgiano B, Nésa M P, Penfold P, Johnson D, Feizi T, Cambillau C

机构信息

Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, Faculté de Médecine Secteur-Nord, Marseille, France.

出版信息

J Mol Biol. 1994 Jan 14;235(2):787-9. doi: 10.1006/jmbi.1994.1034.

DOI:10.1006/jmbi.1994.1034

PMID:8289301

Abstract

The soluble 14 kDa beta-galactoside-binding lectin from bovine heart, a member of the S-type lectin family, has been crystallized in a form suitable for X-ray diffraction analysis. The crystals, in the absence of a saccharide ligand, diffract beyond 2.5 A resolution. They are obtained from polyethylene glycol 6000 at pH 6.0. Crystals grow as monoclinic plates, space group P2(1), with cell dimensions: a = 35.47 A, b = 64.33 A, c = 58.78 A and beta = 91.7 degrees. The asymmetric unit contains two molecules related by a 2-fold non-crystallographic axis. Two lectin monomers in the asymmetric unit give a Vm of 2.4 A3/Da, i.e. a solvent content of approximately 50%. The complex of lectin with the saccharide ligand, N-acetyllactosamine, crystallizes in the space group P2(1)2(1)2 with cell dimensions: a = 63.55 A, b = 82.13 A and c = 62.39 A. Crystals of this complex diffract beyond 2.0 A resolution. Two complexes in the asymmetric unit lead to a Vm value of 2.8 A3/Da (57% solvent).

摘要

牛心可溶性14 kDaβ-半乳糖苷结合凝集素是S型凝集素家族的成员，已结晶成适合X射线衍射分析的形式。在没有糖类配体的情况下，这些晶体的衍射分辨率超过2.5 Å。它们是在pH 6.0条件下从聚乙二醇6000中获得的。晶体生长为单斜板状，空间群为P2(1)，晶胞参数为：a = 35.47 Å，b = 64.33 Å，c = 58.78 Å，β = 91.7°。不对称单元包含通过二重非结晶轴相关的两个分子。不对称单元中的两个凝集素单体的Vm为2.4 Å3/Da，即溶剂含量约为50%。凝集素与糖类配体N-乙酰乳糖胺的复合物在空间群P2(1)2(1)2中结晶，晶胞参数为：a = 63.55 Å，b = 82.13 Å，c = 62.39 Å。该复合物的晶体衍射分辨率超过2.0 Å。不对称单元中的两个复合物导致Vm值为2.8 Å3/Da（57%溶剂）。