Perkins L A, Cain L D
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston, USA.
Int J Dev Neurosci. 1995 Feb;13(1):51-61. doi: 10.1016/0736-5748(94)00066-c.
Basic fibroblast growth factor (bFGF) is found in high concentrations in the mammalian central nervous system. It is a mitogen for glia and it influences the development and survival of specific populations of neurons. In this study, we investigated the effect of various concentrations of bFGF on the survival of embryonic and postnatal cholinergic basal forebrain neurons plated at low and high density in the presence and absence of glia. We observed that 50 and 100 ng/ml of bFGF increased the survival of embryonic cholinergic neurons plated at high density. This effect was observed only in the presence of glia. Lower concentrations of 10 and 20 ng/ml had no effect on cholinergic neuronal survival. The number of GFAP (glial fibrillary acidic protein)-positive cells in high-density embryonic cultures was increased by all concentrations of bFGF. In low-density embryonic cultures, an increase in cholinergic neuron survival was observed at concentrations ranging from 20 to 100 ng/ml. The number of GFAP-positive cells in low-density cultures was also increased by all concentrations of bFGF. Similar to low-density embryonic cultures, the survival of cholinergic neurons from postnatal day 2 cultures was significantly increased in the presence of glia at concentrations of 20, 50 and 100 ng/ml of bFGF. Postnatal glia was affected by all concentrations of bFGF, as was observed in embryonic cultures. This study indicates that high concentrations of bFGF can influence cholinergic neuronal survival by stimulating and increasing glia, which may produce factor(s) that are necessary for cholinergic neuron survival.
碱性成纤维细胞生长因子（bFGF）在哺乳动物中枢神经系统中含量很高。它是一种胶质细胞促分裂原，影响特定神经元群体的发育和存活。在本研究中，我们调查了不同浓度的bFGF在有或无胶质细胞存在的情况下，对低密度和高密度接种的胚胎及出生后胆碱能基底前脑神经元存活的影响。我们观察到，50和100 ng/ml的bFGF可提高高密度接种的胚胎胆碱能神经元的存活率。这种效应仅在有胶质细胞存在时观察到。较低浓度的10和20 ng/ml对胆碱能神经元存活没有影响。所有浓度的bFGF均使高密度胚胎培养物中胶质纤维酸性蛋白（GFAP）阳性细胞数量增加。在低密度胚胎培养物中，20至100 ng/ml浓度范围内的bFGF可使胆碱能神经元存活率增加。所有浓度的bFGF也使低密度培养物中GFAP阳性细胞数量增加。与低密度胚胎培养物相似，在有胶质细胞存在的情况下，出生后第2天培养物中的胆碱能神经元在20、50和100 ng/ml的bFGF浓度下存活率显著增加。如在胚胎培养物中观察到的那样，出生后的胶质细胞受到所有浓度bFGF的影响。本研究表明，高浓度的bFGF可通过刺激和增加胶质细胞来影响胆碱能神经元的存活，胶质细胞可能产生胆碱能神经元存活所需的因子。
