Gajdosová E, Kovácová E, Toman R, Skultéty L, Lukácová M, Kazár J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Acta Virol. 1994 Dec;38(6):339-44.
The immunogenicity and protective efficacy of the phase I and phase II Coxiella burnetii whole cells (Cb I and Cb II) and their outer membrane components (OMC), i.e. phase I trichloroacetic acid extract (TCAE), phase I 29 K protein (PRO), phase I and II lipopolysaccharides (LPS I, LPS II), polysaccharides (PS I, PS II), and lipid A (LA I, LA II), were compared. The highest immune response was observed in BALB/c mice by Cb I in both humoral immunity and lymphocyte transformation assays, and in the protective effect as well. The immune response was also significant by Cb II, but their protective capacity was low. The OMC reacted variously. Only TCAE and PRO gave a high value of humoral immunity evaluated by the serological methods. All OMC reacted in the haemolytic plaque assay giving different responses. Lymphoproliferation of splenocytes was positive with all OMC using both Cb I and Cb II antigens with the exception of PS I and PS II in the case of Cb II antigen. The induction of protection against infectious Cb I was demonstrated after immunization with TCAE, PRO, and LPS I. Other OMC did not induce protection against this agent.
比较了Ⅰ期和Ⅱ期伯纳特柯克斯体全细胞（CbⅠ和CbⅡ）及其外膜成分（OMC）的免疫原性和保护效力，即Ⅰ期三氯乙酸提取物（TCAE）、Ⅰ期29K蛋白（PRO）、Ⅰ期和Ⅱ期脂多糖（LPSⅠ、LPSⅡ）、多糖（PSⅠ、PSⅡ）以及脂质A（LAⅠ、LAⅡ）。在体液免疫和淋巴细胞转化试验中，CbⅠ在BALB/c小鼠中均观察到最高的免疫反应，在保护作用方面也是如此。CbⅡ引起的免疫反应也很显著，但其保护能力较低。OMC的反应各不相同。只有TCAE和PRO通过血清学方法评估时具有较高的体液免疫值。所有OMC在溶血空斑试验中均有反应，呈现不同的结果。使用CbⅠ和CbⅡ抗原时，除了CbⅡ抗原情况下的PSⅠ和PSⅡ外，所有OMC均使脾细胞的淋巴细胞增殖呈阳性。在用TCAE、PRO和LPSⅠ免疫后，证明了对感染性CbⅠ有保护作用的诱导。其他OMC未诱导对该病原体的保护作用。
