Zhang Y X, Zhi N, Yu S R, Li Q J, Yu G Q, Zhang X
Department of Microbiology, Third Military Medical College, Sichuan Province, People's Republic of China.
Acta Virol. 1994 Dec;38(6):327-32.
A 67 K outer membrane protein (OMP) isolated from phase I Coxiella burnetii QiYi strain was purified with monoclonal antibodies (MoAb) coupled to CNBr-Sepharose 4B. Chemical analyses of the 67 K protein showed that it contained seventeen kinds of amino acids and no LPS. The immunogenicity and protectivity of the 67 K protein against C. burnetii was evaluated in mice and guinea pigs by in vitro lymphocyte proliferation assay, delayed-type skin test, antibody conversion rate, and immunization and challenge tests. Intraperitoneal injection of the 67 K protein resulted in antibody production against phase I and II whole cell antigens. The anti-67 K antibody conversion rate was found to be 100% in mice and guinea pigs as well. Lymphocytes were responses in vitro to specific antigen. In addition, delayed-type hypersensitivity appeared two weeks after immunization with the 67 K protein. Moreover, 100% of mice and guinea pigs inoculated with the 67 K protein were protected against a challenge with 10(3) ID50 virulent C. burnetii. In conclusion, these results demonstrate that the 67 K OMP elicits in vivo and in vitro both B cell-mediated and T cell-mediated immunity in mice and guinea pigs. Thus the 67 K protein is a candidate for an effective subunit vaccine against Q fever.
从I期伯纳特立克次体Qiyi菌株中分离出的一种67K外膜蛋白（OMP），用偶联到溴化氰活化的琼脂糖凝胶4B上的单克隆抗体（MoAb）进行纯化。对该67K蛋白的化学分析表明，它含有十七种氨基酸且不含脂多糖。通过体外淋巴细胞增殖试验、迟发型皮肤试验、抗体转化率以及免疫和攻毒试验，在小鼠和豚鼠中评估了该67K蛋白对伯纳特立克次体的免疫原性和保护性。腹腔注射该67K蛋白可诱导产生针对I期和II期全细胞抗原的抗体。在小鼠和豚鼠中，抗67K抗体转化率均为100%。淋巴细胞在体外对特异性抗原产生反应。此外，用67K蛋白免疫两周后出现迟发型超敏反应。而且，接种67K蛋白的小鼠和豚鼠中有100%受到保护，免受10(3) ID50毒力的伯纳特立克次体的攻击。总之，这些结果表明，67K OMP在小鼠和豚鼠体内外均可引发B细胞介导和T细胞介导的免疫反应。因此，67K蛋白是一种有效的Q热亚单位疫苗候选物。
