粘质沙雷氏菌A组（噬菌体尾）细菌素的研究。V. I亚组和II亚组细菌素的血清学特征

Studies on group A (phage tail) bacteriocins of Serratia marcescens. V. Serological characterization of subgroup I and II bacteriocins.

作者信息

Traub W H, Kleber I

出版信息

Zentralbl Bakteriol Orig A. 1976 May;234(4):521-7.

Abstract

Neutralization tests with rabbit hyperimmune sera revealed a close, if not identical, serological relationship among 7 group A (phage tail) bacteriocins of Serratia marcescens of subgroup I, and among 3 phage tail bacteriocins of subgroup II, respectively. On the other hand, subgroup I and II phage tail bacteriocins were found to be serologically unrelated, as determined with neutralization tests and Ouchterlony immunodiffusion experiments. Immunoelectrophoretic tests, employing a representive phage tail bacteriocin of each of the two subgroups, disclosed the electrophoretic mobility of bacteriocin no. 5 (subgroup I), whereas bacteriocin no. 16 (subgroup II) remained stationary. Thus, two additional differential criteria, i.e., differences in antigenicity and electrophoretic mobility, were obtained for the characterization of subgroup I and II group A (phage tail) bacteriocins of S. marcescens.

摘要

用兔超免疫血清进行的中和试验表明，粘质沙雷氏菌I亚组的7种A群（噬菌体尾）细菌素之间以及II亚组的3种噬菌体尾细菌素之间分别存在密切的血清学关系，即便不是完全相同。另一方面，通过中和试验和双向免疫扩散实验确定，I亚组和II亚组的噬菌体尾细菌素在血清学上没有关系。使用两个亚组各自具有代表性的噬菌体尾细菌素进行免疫电泳试验，揭示了5号细菌素（I亚组）的电泳迁移率，而16号细菌素（II亚组）则保持不动。因此，获得了另外两个鉴别标准，即抗原性和电泳迁移率的差异，用于鉴定粘质沙雷氏菌I亚组和II亚组的A群（噬菌体尾）细菌素。