Nakamura T, Kusata T, Matsumoto H, Sato J
Department of Industrial Chemistry, School of Science and Technology, Meiji University, Kawasaki, Japan.
Anal Biochem. 1995 Apr 10;226(2):256-62. doi: 10.1006/abio.1995.1223.
A method of graphite furnace atomic absorption spectrometry (GFAAS) with a direct atomization technique has been developed for the determination of trace amounts of Cd and Pb in human teeth and artificial teeth. The samples were ground to a particle size of less than 10 microns and mixed with the same amount of graphite powder. One-half to 2 mg of mixed sample was weighed in a tared graphite cup and atomized in a cup-type graphite furnace according to the heating program that was established. Absorbances were determined by integration of the spectral lines in the absorbance vs time spectrum. Calibration was affected using aqueous standard solutions. The results for six human tooth samples and two artificial tooth samples were in good agreement with the values obtained by GFAAS for dissolved samples. The relative standard deviations were 3.1-12% (n = 10) for Cd (19-95 ng g-3) and 0.9-8.1% (n = 10) for Pb (1.43-6.32 micrograms g-3). The determination limits corresponding to twice the standard deviation (sigma, n = 10) for blank measurements were 13 pg for Cd and 53 pg for Pb.
已开发出一种采用直接雾化技术的石墨炉原子吸收光谱法（GFAAS），用于测定人牙和人造牙中的痕量镉和铅。将样品研磨至粒径小于10微米，并与等量的石墨粉混合。在已称重的石墨杯中称取0.5至2毫克混合样品，并根据既定的加热程序在杯式石墨炉中进行雾化。通过对吸光度与时间谱中的谱线进行积分来测定吸光度。使用水溶液标准溶液进行校准。六个人类牙齿样品和两个人造牙齿样品的结果与通过GFAAS对溶解样品获得的值高度一致。镉（19 - 95纳克/克³）的相对标准偏差为3.1 - 12%（n = 10），铅（1.43 - 6.32微克/克³）的相对标准偏差为0.9 - 8.1%（n = 10）。空白测量中对应两倍标准偏差（σ，n = 10）的测定限，镉为13皮克，铅为53皮克。
