Kennedy S W, Jones S P, Bastien L J
Canadian Wildlife Service, National Wildlife Research Centre, Hull, Quebec.
Anal Biochem. 1995 Apr 10;226(2):362-70. doi: 10.1006/abio.1995.1237.
An efficient method for measuring polychlorinated biphenyl-mediated induction of cytochrome P4501A, using the ethoxyresorufin-O-deethylase (EROD) assay, and total porphyrins in chicken embryo hepatocyte cultures is described. Hepatocytes were cultured in 48-well plates, assays were carried out within the wells, and concentrations of the product of the EROD reaction (resorufin), porphyrins, and total proteins were measured in the same wells with a fluorescence plate reader. The method differs from previous methods developed in this laboratory in that three fluorophors were measured in the same well rather than two.
本文描述了一种利用乙氧异吩唑酮 -O-脱乙基酶（EROD）测定法来测量多氯联苯介导的鸡胚肝细胞培养物中细胞色素P4501A诱导以及总卟啉的有效方法。肝细胞在48孔板中培养，测定在孔内进行，用荧光酶标仪在同一孔中测量EROD反应产物（试卤灵）、卟啉和总蛋白的浓度。该方法与本实验室之前开发的方法不同之处在于，在同一孔中测量三种荧光团而非两种。
