Tanimura N, Tsukamoto K, Nakamura K, Narita M, Maeda M
National Institute of Animal Health, Ibaraki, Japan.
Avian Dis. 1995 Jan-Mar;39(1):9-20.
Highly pathogenic infectious bursal disease virus (IBDV) strains (Ehime/91, DV86) and a moderately pathogenic strain (J1) were compared in order to clarify the association between the pathogenicity of IBDV and viral antigen distribution. Virus target cells in the bursa, thymus, spleen, and bone marrow were examined by transmission electron microscopy. Although all strains caused similar bursal atrophy, the highly pathogenic strains brought about a greater decrease in the thymic weight index and more severe lesions in the cecal tonsil, thymus, spleen, and bone marrow. Immunohistochemical detection of IBDV antigen in tissues from chickens infected with Ehime/91 and DV86 strains showed a higher frequency of antigen-positive cells in the spleen and bone marrow. Transmission electron microscopy indicated the presence of viral particles in the cytoplasm of epithelial reticular cells in the thymus and monocytes in the bone marrow. The results show that pathogenicity of field strains of IBDV correlates with lesion production in non-bursal lymphopoietic organs. The results also suggest that pathogenicity of IBDV may be associated with virus antigen distribution in non-bursal lymphopoietic organs.
为了阐明传染性法氏囊病病毒（IBDV）的致病性与病毒抗原分布之间的关联，对高致病性IBDV毒株（爱媛/91、DV86）和一株中等致病性毒株（J1）进行了比较。通过透射电子显微镜检查了法氏囊、胸腺、脾脏和骨髓中的病毒靶细胞。尽管所有毒株均引起相似的法氏囊萎缩，但高致病性毒株导致胸腺重量指数下降幅度更大，盲肠扁桃体、胸腺、脾脏和骨髓中的病变更严重。对感染爱媛/91和DV86毒株的鸡组织进行IBDV抗原的免疫组织化学检测显示，脾脏和骨髓中抗原阳性细胞的频率更高。透射电子显微镜表明，胸腺上皮网状细胞的细胞质和骨髓单核细胞中存在病毒颗粒。结果表明，IBDV野毒株的致病性与非法氏囊淋巴造血器官中的病变产生相关。结果还表明，IBDV的致病性可能与非法氏囊淋巴造血器官中的病毒抗原分布有关。
