IRTA17和IRTA21的建立与鉴定，两种具有t(16;21)易位的新型急性非淋巴细胞白血病细胞系。

Establishment and characterization of IRTA17 and IRTA21, two novel acute non-lymphocytic leukaemia cell lines with t(16;21) translocation.

作者信息

Hiyoshi M, Yamane T, Hirai M, Tagawa S, Hattori H, Nakao Y, Yasui Y, Koh K R, Hino M, Tatsumi N

机构信息

Division of Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

出版信息

Br J Haematol. 1995 Jun;90(2):417-24. doi: 10.1111/j.1365-2141.1995.tb05168.x.

DOI:10.1111/j.1365-2141.1995.tb05168.x

PMID:7794765

Abstract

The t(16;21)(p11;q22) translocation is an infrequent chromosomal abnormality, but seems specific to acute non-lymphocytic leukaemia (ANLL). We established two cell lines with t(16;21)(p11;q22) from the bone marrow of a patient with ANL in relapse. Their morphological, karyotypic, immunohistochemical and genetic features are examined. Although both cell lines show monocytoid features morphologically, they express only CD13 (My7) and CD34, and neither expressed monocytoid or lymphoid markers. Reverse transcription-polymerase chain reaction showed that both cell lines expressed a similar TLS-ERG chimaeric mRNA as a result of the t(16;21)(p11;q22) translocation. As far as we know, there is no report of a leukaemia cell line with t(16;21). These cell lines represent a useful tool for leukaemia research.

摘要

t(16;21)(p11;q22)易位是一种罕见的染色体异常，但似乎是急性非淋巴细胞白血病（ANLL）所特有的。我们从一名复发的急性非淋巴细胞白血病患者的骨髓中建立了两株具有t(16;21)(p11;q22)的细胞系。对它们的形态学、核型、免疫组织化学和遗传学特征进行了检测。虽然两株细胞系在形态上均显示单核细胞样特征，但它们仅表达CD13（My7）和CD34，均未表达单核细胞样或淋巴细胞标志物。逆转录-聚合酶链反应显示，由于t(16;21)(p11;q22)易位，两株细胞系均表达相似的TLS-ERG嵌合mRNA。据我们所知，尚无具有t(16;21)的白血病细胞系的报道。这些细胞系是白血病研究的有用工具。