巨细胞病毒肺炎患者血清中检测到巨细胞病毒血症。
CMV viraemia demonstrated in the serum of a patient with cytomegalovirus pneumonia.
作者信息
Watanabe N, Takeda M, Ishigaki S, Tsuji N, Sakamaki S, Kato J, Kohda K, Mori Y, Niitsu Y
机构信息
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
出版信息
Br J Haematol. 1995 Jun;90(2):457-8. doi: 10.1111/j.1365-2141.1995.tb05174.x.
We attempted to demonstrate the expression of cytomegalovirus (CMV) particles in the serum of an acute lymphocytic leukaemia patient with CMV pneumonia. The serum sample was applied to an affinity column coupled with human monoclonal antibody C23 which recognizes the envelope glycoproteins of CMV virus and neutralizes the viral activity. The DNA obtained from each fraction was amplified by double polymerase chain reaction (PCR) and analysed by gel electrophoresis. Bands were clearly observed in the eluted fraction. These results strongly suggest that CMV particles exist in the sera of patients with CMV pneumonia.
我们试图证明巨细胞病毒（CMV）颗粒在一名患有CMV肺炎的急性淋巴细胞白血病患者血清中的表达。将血清样本应用于与人类单克隆抗体C23偶联的亲和柱，该抗体识别CMV病毒的包膜糖蛋白并中和病毒活性。通过双重聚合酶链反应（PCR）扩增从每个馏分中获得的DNA，并通过凝胶电泳进行分析。在洗脱馏分中清晰观察到条带。这些结果有力地表明CMV颗粒存在于CMV肺炎患者的血清中。
Zotero 插件