Paradiso A, Barletta A, Prete F, Pellecchia A, Romagno D, Lucchese G, Benvestito V, Zito F A, Russo S, De Lena M
Ospedale Oncologico, IRCCS, Università di Bari, Italy.
Ital J Gastroenterol. 1995 Jan-Feb;27(1):8-12.
In the search for parameters that can indicate changes in the behaviour of liver tissue from normal to chronic to neoplastic disease, DNA content by FCM (ploidy and percent of 4N cells) and morphobiological characteristics were investigated in fresh liver specimens of 16 patients with normal liver, 21 with persistent hepatitis (CPH), 23 with chronic active hepatitis (CAH), 17 with cirrhosis, and 13 with hepatocellular carcinoma (HCC). Aneuploidy was mostly found in HCC specimens (54%), whereas the percentage of 4N peak decreased in chronic hepatitis and cirrhosis patients but increased to 11.09% in HCC samples (r = -0.02; p = 0.05). Finally, the binuclearity rate decreased gradually from normal to flogistic to HCC specimens. The 4N peak and the binuclearity rate were closely correlated in non-HCC (p = 0.0006, by T-test) but not in HCC samples. Only DNA ploidy and the binuclearity rate have been confirmed as being significantly and independently related to the histology of liver tissue by multivariate regression analysis.
为了寻找能够指示肝组织从正常状态转变为慢性疾病再到肿瘤性疾病过程中行为变化的参数，我们对16例正常肝脏患者、21例持续性肝炎（CPH）患者、23例慢性活动性肝炎（CAH）患者、17例肝硬化患者和13例肝细胞癌（HCC）患者的新鲜肝脏标本进行了流式细胞术检测DNA含量（倍体和4N细胞百分比）以及形态生物学特征的研究。非整倍体大多出现在HCC标本中（54%），而4N峰的百分比在慢性肝炎和肝硬化患者中降低，但在HCC样本中增加至11.09%（r = -0.02；p = 0.05）。最后，双核率从正常标本到炎症标本再到HCC标本逐渐降低。4N峰与双核率在非HCC标本中密切相关（经T检验，p = 0.0006），但在HCC样本中并非如此。通过多变量回归分析，只有DNA倍体和双核率被证实与肝组织的组织学显著且独立相关。
